Online Course: BI231 Next Generation Sequencing Data Analysis


                        BI231 NGS Data Analysis
                          June 27-July 1, 2011
                      Online at Bioinformatics.Org


This course teaches biologists how to analyze data from Next Generation Sequencing (NGS) platforms. Topics to be covered include the description of sequencing strategies and platforms, experiment types, data formats, and command line tools for various workflows, such as quantification of transcripts, alternative splice forms, copy number variants, single nucleotide polymorphisms, etc.

Shailender Nagpal, M.Sc. is a data analysis consultant in the biotechnology and pharmaceutical industries and specializes in the interpretation of data generated from various platforms in drug discovery, such as genomics and proteomics.


The course is divided into 4 sessions, with live lectures tentatively scheduled for 12:00 PM to 1:30 PM US EDT, Monday through Thursday (Friday will be a reserve day). Homework assignments will involve running computationally intensive tools.

Session 1:
Fundamentals of NGS technologies – strategies, platforms and types of experiments that can be done (whole genome, transcriptome, targeted, ChIP-Seq, etc.)

Session 2:
Data formats and overview of algorithms, workflows and command line tools

Session 3:
Whole genome sequencing workflow used for CNV and SNP discovery

Session 4:
Transcriptomics workflow for quantifying abundance of transcripts and alternative splicing junctions

Course attendees should have access to a powerful Linux/Unix workstation to run the examples in this course. Minimum requirements: Dual-CPU, quad-core 2 GHz processor or higher, 32 GB RAM, 200 GB free disk space. Attendees can optionally subscribe to our server for 1 month for an additional $100 lab fee.

For-profit & government: $1,000 USD
Non-profit & self-pay: $600 USD

Additional discounts are available as follows:
* Professional members: 20% discount
* Limited scholarships may also be available for those in low-income economies (please inquire).

For more information and registration, please visit: contact


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